Need to Object Again?

Motions in limine are a good practice on personal injury cases.  What happens when it is denied and the issue comes up in trial?  Do you have to object again?  Mississippi does not seemed to have addressed the issue.  A court in Wyoming has said yes that another objection needs to be made  in Hicks v. Zondag, 2014 WY 16 (Jan. 28, 2014) in order to preserve the issue for appeal.  If the second objection is not made, it is waived on appeal according to this case.  Tennessee has a case with similar language in it.  I would expect Mississippi Courts to make a similar ruling.